Cloning a Gene from Arabidopsis

Ben Carter, 3/10/15

 WORK IN PROGRESS 

Materials
DNeasy Plant Mini Kit (Qiagen Cat# 69104)

Phusion High-Fidelity DNA Polymerase (NEB Cat# M0530S)

Visual Violet Gel Kit (Amresco Cat# N733-KIT)

NucleoSpin Gel and PCR Clean-up (Machery-Nagel Cat#740609.50)

pENTR/D-TOPO Cloning Kit (Life Technologies Cat# K2435-20)

LR Clonase II Plus (Life Technologies Cat# 12538-120)

Genomic DNA Extraction using DNeasy Plant Mini kit

 * 1) Harvest